Brian Stevenson, Ph.D.

Research Description: 

Our research focuses on understanding the mechanisms by which pathogenic bacteria sense their environments and respond by regulating gene expression, and the functions of bacterial surface proteins during infection processes. These studies have identified several novel regulatory factors in the Lyme diease agent, Borrelia burgdorferi, and other pathogens. Surprisingly, some of these regulatory proteins are produced by many other bacterial species, but had never before been studied. As a result, our research is having impacts on the understanding of a broad range of bacterial pathogens. In addition, one B. burgdorferi protein we discovered, named BpuR, is homologous to human proteins that play significant roles in human diseases such as cancers and AIDS.

Lyme disease is caused by the spirochetal bacterium Borrelia burgdorferi. Spread by the bites of certain tick species, it is the most common arthropod-borne disease of humans in the United States and many other temperate regions of the world. B. burgdorferi has evolved mechanisms by which it can infect both mammalian and arthropod hosts, and be efficiently transmitted between these two very different types of animals.  To do so, B. burgdorferi senses its environment and responds accordingly by producing proteins appropriate for each step in the infectious cycle. We recently discovered that B. burgdorferi uses its own growth/division rate and the supply of certain nutrients to determine its position in the tick-mammal infectious cycle, and thus "knows" when to express virulence factors.

Similar approaches are being taken to investigate pathogenic properties of other bacteria, including Treponema pallidum (syphilis), Leptospira interrogans (leptospirosis) and Borrelia miyamotoi (hard-tick relapsing fever).

Grants: 

Research in the Stevenson lab is currently funded by U.S. NIH grants R21 AI106260, R21 AI120602, R21 AI126883, R03 AI113648, and R03 AI126252.

Selected Publications: 

Publications:

Journal Articles
Edited Books
Invited Reviews
Book Chapters

 

Journal Articles:

Arnold, W.K., C.R. Savage, C.A. Brissette, J. Seshu, J. Livny, and B. Stevenson (2016) RNA-Seq of Borrelia burgdorferi in multiple phases of growth reveals insights into the dynamics of gene expression, transcriptome architecture, and noncoding RNAs. PLoS One 11: e0164165. [Abstract]

Byram, R., R.A. Gaultney, A.M. Floden, C. Hellekson, B.L. Stone, A. Bowman, B. Stevenson, B.J. Johnson, C.A. Brissette (2015) Borrelia burgdorferi RevA significantly affects pathogenicity and host response in the mouse model of Lyme disease. Infect Immun. 89: 3675-3683. [Abstract]

Savage, C.R., W.K. Arnold, A. Gjevre-Nail, B.J. Koestler, E.L. Bruger, J.R. Barker, C.M. Waters, and B. Stevenson (2015) Intracellular concentrations of Borrelia burgdorferi cyclic di-AMP are not changed by altered expression of the CdaA synthase. PLoS One 10: e0125440. [Abstract]

Arnold, W.K., C.R. Savage, A.D. Antonicello, and B. Stevenson (2015) Apparent role for Borrelia burgdorferi LuxS during mammalian infection. Infect. Immun. 83: 1347-1353. [Abstract]

Moniuszko, A., C. Rückert, M.P. Alberdi, G. Barry, B. Stevenson, J.K. Fazakerley, A. Kohl, and L. Bell-Sakyi (2014) Co-infection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens. Ticks Tick-Borne Dis. 5:415-22

Graves, C.J., V.I.D. Ros, B. Stevenson, P.D. Sniegowski, and D. Brisson (2013) Natural selection promotes antigenic evolvability, PLoS Pathogens, 9: e1003766. doi:10.1371/journal.ppat.1003766. [Abstract]

Jutras, B.L., G.S. Jones, A. Verma, N.A. Brown, A.D. Antonicello, A.M. Chenail, and B. Stevenson (2013) Post-transcriptional autoregulation of the Lyme disease bacterium¹s BpuR DNA/RNA-binding protein. J. Bacteriol.195:4915-4923. [Abstract]

Jutras, B.L., A.M. Chenail, D.W. Carroll, M.C. Miller, H. Zhu, A. Bowman, and B. Stevenson (2013) Bpur, the Lyme disease spirochete¹s PUR-domain protein: identification as a transcriptional modulator and characterization of nucleic acid interactions. J. Biol. Chem. 288:26220-26234. [Abstract]

Koenigs, A., C. Hammerschmidt, B.L. Jutras, D. Pogoryelov, D.Barthel, C. Skerka, D. Kugelstadt, R. Wallich, B. Stevenson, P.F. Zipfel, P. Kraiczy (2013) BBA70 of Borrelia burgdorferi is a novel plasminogen-binding protein, J. Biol. Chem. 288: 25229-25243. [Abstract]

Jutras, B.L., A.M. Chenail, C.L. Rowland, D. Carroll, M.C. Miller, T. Bykowski, and B. Stevenson (2013) Eubacterial SpoVG homologs constitute a new family of site-specific DNA-binding proteins. PLoS One 8:e66683. [Abstract]

Brisson, D., W. Zhou, B.L. Jutras, S. Casjens, and B. Stevenson (2013) Distribution of cp32 prophages among Lyme disease-causing spirochetes and natural diversity of their lipoprotein-encoding erp loci. Appl. Env. Microbiol. 79: 4115-4128. [Abstract]

Jutras, B.L., A.M. Chenail, and B. Stevenson (2013) Changes in bacterial growth rate govern expression of the Borrelia burgdorferi OspC and Erp infection-associated surface proteins. J. Bacteriol. 195: 757-764. [Abstract]

Chenail, A.M, B.L. Jutras, C.A. Adams, L.H. Burns, A. Bowman, A. Verma and B. Stevenson (2012) Borrelia burgdorferi cp32 BpaB modulates expression of the prophage NucP nuclease and SsbP single-stranded DNA-binding protein. J. Bacteriol. 194: 4570-4578. [Abstract]

Jutras, B.L., A. Bowman, C.A. Brissette, C.A. Adams, A. Verma, A.M. Chenail, and B. Stevenson (2012) EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete. J. Bacteriol. 194: 3395-3406. [Abstract]

Jutras, B.L., A. Verma, C.A. Adams, C.A. Brissette, L.H. Burns, C.R. Whetstine, A. Bowman, A.M. Chenail, W.R. Zückert, and B. Stevenson (2012) BpaB and EbfC DNA-binding proteins regulate production of the Lyme disease spirochete's infection-associated Erp surface proteins. J. Bacteriol. 194: 778-786. [Abstract]

Verma, A., J. Matsunaga, S. Artiushin, M. Pinne, D.J. Houwers, D.A. Haake, B. Stevenson and J.F. Timoney (2012) Antibodies to a novel leptospiral protein, LruC, in the eye fluids and sera of horses with Leptospira-associated uveitis. Clin. Vac. Immunol. 19:452-456. [Abstract]

Hammerschmidt, C., T. Hallström, C. Skerka, R. Wallich, B. Stevenson, P. Zipfel and P. Kraiczy (2012) Contribution of the infection-associated complement regulator acquiring surface protein 4 (ErpC) to complement resistance of Borrelia burgdorferi. Clin. Dev. Immunol. 2012: 349657 [Abstract]

Jutras, B.L., A. Verma, and B. Stevenson (2012)  Identification of novel DNA binding proteins using DNA affinity chromatography-pulldown. Curr. Protocols Microbiol. 1F.1.1-13. [Abstract]

Burns, L.H., C.A. Adams, S.P. Riley, B.L. Jutras, A. Bowman, A.M. Chenail, A.E. Cooley, L.A. Haselhorst, A.M. Moore, K. Babb, M.G. Fried, and B. Stevenson (2010) BpaB, a novel protein encoded by the Lyme disease spirochete¹s cp32 prophages, binds to erp Operator 2 DNA.  Nucleic Acids Res., 38: 5443-5455. [Article]

Verma, A., P. Kumar, K. Babb, J.F. Timoney, B. Stevenson (2010) Cross-reactivity of antibodies against leptospiral recurrent uveitis-associated proteins A and B (LruA and LruB) with eye proteins. PLoS Neglected Trop. Dis., 4: e778. [Article]

Sindhava, V., M.E. Woodman, B. Stevenson, and S. Bondada (2010) Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection.  PLoS One 5: e11445.  [Article]

Siegel, C., T. Hallström, C. Skerka, H. Eberhardt, B. Uzonyi, T. Beckhaus, M. Karas, R. Wallich, B. Stevenson, P.F. Zipfel, and P. Kraiczy (2010) Complement factor H-related proteins CFHR2 and CFHR5 represent novel ligands for the infection-associated CRASP proteins of Borrelia burgdorferi.  PLoS One 5: e13519. [Article]

Verma, A., C.A. Brissette, A.A. Bowman, S.T. Shah, P.F. Zipfel and B. Stevenson (2010) Leptospiral endostatin-like protein A (LenA) is a bacterial cell-surface receptor for human plasminogen.  Infect. Immun. 78:2053-2059. [Abstract]

Brissette, C.A., E. Rossmann, A. Bowman, A.E. Cooley, S.P. Riley, K.-P. Hunfeld, M. Bechtel, P. Kraiczy, and B. Stevenson (2010)  The borrelial fibronectin-binding protein RevA is an early antigen of human Lyme disease. Clin. Vac. Immunol., 17: 274-280. [Abstract]

Seling, A., C. Siegel, V. Fingerle, B.L. Jutras, C.A. Brissette, C. Skerka, R. Wallich, P.F. Zipfel, B. Stevenson, and P. Kraiczy (2010)  Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen.   Infect. Immun., 78: 39-48. [Abstract]

Verma, A., C.A. Brissette, A. Bowman and B. Stevenson (2009)  Borrelia burgdorferi BmpA is a laminin-binding protein.  Infect. Immun., 77:4940-4946. [Abstract]

Woodman, M.E., A.E. Cooley, R. Avdiushko, A. Bowman, M. Botto, R.M. Wooten, N. van Rooijen, D.A. Cohen, and B. Stevenson (2009)  Roles for phagocytic cells and complement in controlling relapsing fever infection. J. Leukoc. Biol. 85: 727-736. [Article]

Brissette, C.A., T. Bykowski, A.E. Cooley, A. Bowman, and B. Stevenson (2009) The Borrelia burgdorferi RevA antigen binds host fibronectin. Infect. Immun. 77: 2802-2812. [Abstract]

Cooley, A.E., S.P. Riley, K. Kral, M.C. Miller, E. DeMoll, M.G. Fried, and B. Stevenson (2009)  DNA-binding by Haemophilus influenzae and Escherichia coli YbaB, members of a widely-distributed bacterial protein family. BMC Microbiol. 9: 137.  [Article]

Riley, S.P., T. Bykowski, A.E. Cooley, L.H. Burns, K. Babb, C.A. Brissette, A. Bowman, M. Rotondi, M.C. Miller, E. DeMoll, K. Lim, M.G. Fried, and B. Stevenson (2009) Borrelia burgdorferi EbfC defines a newly-identified, widespread family of bacterial DNA-binding proteins. Nucleic Acids Res. 37: 1973-1983.  [Abstract]

Brissette, C.A., A. Verma, A. Bowman, A.E. Cooley, and B. Stevenson (2009) The Borrelia burgdorferi outer-surface protein ErpX binds mammalian laminin.  Microbiology 155: 863-872. [Abstract]

Kraiczy, P., C. Hanssen-Hübner, V. Kitiratschky, C. Brenner, S. Besier, V. Brade, M.M. Simon, C. Skerka, P. Roversi, S.M. Lea, B. Stevenson, R. Wallich and P.F. Zipfel (2009) Mutational analyses of the BbCRASP-1 protein of Borrelia burgdorferi identify residues relevant for the architecture and binding of host complement regulators FHL-1 and Factor H.  Int. J. Med. Microbiol. 299: 255-268. [Abstract]

Brissette, C.A., K. Haupt, D. Barthel, A.E. Cooley, A. Bowman, C. Skerka, R. Wallich, P.F. Zipfel, P. Kraiczy and B. Stevenson (2009) The Borrelia burgdorferi infection-associated surface proteins ErpP, ErpA, and ErpC bind human plasminogen. Infect. Immun. 77: 300-306. [Abstract]

Siegel, C., J. Schreiber, K. Haupt, C. Skerka, V. Brade, M.M. Simon, B. Stevenson, R. Wallich, P.F. Zipfel, and P. Kraiczy (2008) Deciphering the ligand-binding sites in the Borrelia burgdorferi complement regulator-acquiring surface protein 2 (BbCRASP-2) required for interactions with the human immune regulators factor H and factor H-like protein-1.  J. Biol. Chem. 283: 34855-34863. [Abstract]

Woodman, M.E., A.E. Cooley, B. Stevenson (2008) Production of outer surface protein A by Borrelia burgdorferi during transmission from infected mammals to feeding ticks is insufficient to trigger OspA seroconversion.  FEMS Immunol. Mol. Microbiol. 54: 277-282. [Abstract]

Verma, A., S.R. Rathinam, C.G. Priya, V.R. Muthukkaruppan, B. Stevenson, and J.F. Timoney (2008) LruA- and LruB-directed antibodies in sera of human leptospiral uveitis patients.  Clin. Vac. Immunol. 15:1019-1023. [Abstract]

Kraiczy, P., A. Seling, C.A. Brissette, E. Rossmann, K.-P. Hunfeld, T. Bykowski, L.H. Burns, M.J. Troese, A.E. Cooley, J.C. Miller, V. Brade, R. Wallich, S. Casjens, B. Stevenson (2008) Antibodies against Borrelia burgdorferi complement regulator-acquiring surface protein 2 (CspZ) as a serological marker of human Lyme disease.  Clin. Vac. Immunol., 15: 484-491. [Abstract]

Stevenson, B., H.A. Choy, M. Pinne, M.L. Rotondi, M.C. Miller, E. DeMoll, P. Kraiczy, A.E. Cooley, T.P. Creamer, M.A. Suchard, C.A. Brissette, A. Verma, and D.A. Haake (2007) Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement.  PLoS ONE 2: e1188, doi:10.1371/journal.pone.0001188. [Article]

Bykowski, M.E. Woodman, A.E. Cooley, C.A. Brissette, V. Brade, R. Wallich, P. Kraiczy and B. Stevenson (2007) Coordinated expression of Borrelia burgdorferi complement regulator-acquiring surface proteins during the Lyme disease spirochete¹s mammal-tick infection cycle. Infect. Immun. 75: 4227-4236. [Abstract]

Riley, S.P., T. Bykowski, K. Babb, K. von Lackum, and B. Stevenson (2007) Genetic and physiological characterization of the Borrelia burgdorferi ORF BB0374-pfs-metK-luxS operon. Microbiology 153: 2304-2311. [Abstract]

Woodman, M.E., A.E. Cooley, J.C. Miller, J.J. Lazarus, K. Tucker, T. Bykowski, M. Botto, J. Hellwage, R.M. Wooten, B. Stevenson (2007) Borrelia burgdorferi binding of host complement regulator factor H is not required for efficient mammalian infection. Infect. Immun. 75: 3131-3139. [Abstract]

von Lackum, K., T. Bykowski, K. Ollison, A.J. Nowalk, J.L. Hughes, J.A. Carroll, W.R. Zückert, and  B. Stevenson (2007) Regulated synthesis of the Borrelia burgdorferi inner-membrane lipoprotein IpLA7 (P22, P22-A) during the Lyme disease spirochaete¹s mammal-tick infectious cycle. Microbiology 153: 1361-1371. [Abstract]

Hartmann, K., C. Corvey, C. Skerka, M. Kirschfink, M. Karas, V. Brade, J.C. Miller, B. Stevenson, R. Wallich, P.F. Zipfel, and P. Kraiczy (2006) Functional characterization of BbCRASP-2, a distinct outer membrane protein of Borrelia burgdorferi that binds host complement regulators factor H and FHL-1.  Mol. Microbiol. 61:1220-1236. [Abstract]

Babb, K., T. Bykowski, S.P. Riley, M.C. Miller, E. DeMoll, and B. Stevenson (2006) Borrelia burgdorferi EbfC, a novel, chromosomally-encoded protein, binds specific DNA sequences adjacent to erp loci on the spirochete’s resident cp32 prophages. J. Bacteriol. 188:4331-4339. [Abstract]

Bykowski, T., K. Babb, K. von Lackum, S.P. Riley, S.J. Norris, and B. Stevenson (2006) Transcriptional regulation of the Borrelia burgdorferi antigenically variable VlsE surface protein during the mammal-tick infectious cycle. J. Bacteriol. 188:4879-4889. [Abstract]

Miller, J.C., and B. Stevenson (2006) Borrelia burgdorferi erp genes are expressed at different levels within tissues of chronically infected mammalian hosts. Int. J. Med. Microbiol. 296 (S1):185-194. [Abstract]

Verma, A., J. Hellwage, S. Artiushin, P.F. Zipfel, P. Kraiczy, J.F. Timoney, and B. Stevenson (2006) LfhA, a novel factor H binding protein of Leptospira interrogans. Infect. Immun. 74:2659-2666 [Abstract]

Miller, J.C., K. von Lackum, M.E. Woodman, and B. Stevenson (2006) Detection of Borrelia burgdorferi gene transcription during mammalian infection using transcriptional fusions that produce green fluorescent protein. Microb. Pathog. 41:43-47. [Abstract]

von Lackum, K., J.C. Miller, S.P. Riley, M.E. Woodman, V. Brade, P. Kraiczy, B. Stevenson and R. Wallich (2005) Borrelia burgdorferi regulates expression of complement regulator-acquiring surface protein 1 during the mammal-tick infection cycle. Infect. Immun. 73:7398-7405 [Abstract]

Miller, J.C., K. Narayan, B. Stevenson, and A.R. Pachner (2005) Expression of Borrelia burgdorferi erp genes during infection of non-human primates. Microb. Pathog. 39:27-33 [Abstract]

Babb, K., K. von Lackum, R.L. Wattier, S.P. Riley, and B. Stevenson (2005) Synthesis of autoinducer-2 by the Lyme disease spirochete, Borrelia burgdorferi. J. Bacteriol. 187:3079-3087 [Abstract]

von Lackum, K., and B. Stevenson (2005) Carbohydrate utilization by the Lyme borreliosis spirochete, Borrelia burgdorferi. FEMS Micrbiol. Lett. 243:173-179 [Abstract]

K. Babb, J.D. McAlister, J.C. Miller, and B. Stevenson (2004) Molecular characterization of Borrelia burgdorferi erp promoter/operator elements. J. Bacteriol. 186: 2745-2756. [Abstract]

Miller, J.C., K. von Lackum, K. Babb, J.D. McAlister, and B. Stevenson (2003) Temporal analysis of Borrelia burgdorferi Erp protein expression throughout the mammal-tick infectious cycle. Infect. Immun. 71:6943-6952. [Abstract]

Kraiczy, P., K. Hartmann, J. Hellwage, C. Skerka, V. Brade, P.F. Zipfel, R. Wallich, and B. Stevenson (2003) Immunological characterization of the complement regulator factor H-binding CRASP and Erp proteins of Borrelia burgdorferi. Int. J. Med. Microbiol. 293 S37: 152-157.

Miller, J.C., and B. Stevenson (2003) Increased expression of Borrelia burgdorferi factor H-binding surface proteins during transmission from ticks to mice. Int. J. Med. Microbiol. 293 S37: 120-125.

Stevenson, B., and J.C. Miller (2003) Intra- and interbacterial genetic exchange of Lyme disease spirochete erp genes generate sequence identity amidst diversity. J. Mol. Evol. 57: 309-324. [Abstract]

Miller, J.C., and B. Stevenson (2003) Immunological and genetic characterization of Borrelia burgdorferi BapA and EppA proteins. Microbiology 149: 1113-1125. [Abstract ]

Iyer, R., O. Kalu, J.E. Purser, S.J. Norris, B. Stevenson and I. Schwartz (2003) Linear and circular plasmid content in Borrelia burgdorferi clinical isolates. Infect. Immun.71: 3699-3706. [Abstract]

El-Hage, N., and B. Stevenson (2002) Simultaneous coexpression of Borrelia burgdorferi Erp proteins occurs through a specific, erp locus-directed regulatory mechanism. J. Bacteriol. 184: 4536-4543.[Abstract]

Stevenson, B. (2002) Borrelia burgdorferi erp (ospE-related) gene sequences remain stable during mammalian infection. Infect. Immun. 70: 5307-5311. [Abstract]

Stevenson, B., and K. Babb (2002) LuxS-mediated quorum sensing in Borrelia burgdorferi, the Lyme disease spirochete. Infect. Immun. 70: 4099-4105. [Abstract]

Mbow, M.L., R.D. Gilmore, Jr., B. Stevenson, W.T. Golde, J. Piesman, and B.J.B. Johnson (2002) Borrelia burgdorferi-specific monoclonal antibodies derived from mice primed with Lyme disease spirochete-infected Ixodes scapularis ticks. Hybridoma Hybridomics 21: 179-182. [Abstract]

Stevenson, B., N. El-Hage, M.A. Hines, J.C. Miller, and K. Babb (2002) Differential binding of host complement inhibitor factor H by Borrelia burgdorferi Erp surface proteins: a possible mechanism underlying the expansive host range of Lyme disease spirochetes. Infect. Immun. 70: 491-497. [Abstract]

Carroll, J.A., N. El-Hage, J.C. Miller, K. Babb, and B. Stevenson (2001) Borrelia burgdorferi RevA antigen is a surface-exposed outer membrane protein whose expression is regulated in response to environmental temperature and pH. Infect. Immun. 69: 5286-5293. [Abstract]

Gilmore, R.D., Jr., M.L. Mbow, and B. Stevenson (2001) Analysis of Borrelia burgdorferi gene expression during life cycle phases of the tick vector Ixodes scapularis. Microbes Infect. 3: 799-808. [Abstract]

Babb, K., N. El-Hage, J.C. Miller, J.A. Carroll, and B. Stevenson (2001) Distinct regulatory pathways control expression of Borrelia burgdorferi infection-associated OspC and Erp surface proteins. Infect. Immun. 69: 4146-4153. [Abstract]

El-Hage, N., K. Babb, J.A. Carroll, N. Lindstrom, E.R. Fischer, J.C. Miller, R.D. Gilmore, Jr., M.L. Mbow, and B. Stevenson (2001) Surface exposure and protease insensitivity of Borrelia burgdorferi Erp (OspEF-related) lipoproteins. Microbiology 147: 821-830. [Abstract]

Nally, J.E., J.F. Timoney, and B. Stevenson (2001) Temperature-regulated protein synthesis by Leptospira interrogans. Infect. Immun. 69: 400-404. [Abstract]

Miller, J.C., J.L. Bono, K. Babb, N. El-Hage, S. Casjens, and B. Stevenson (2000) A second allele of eppA in Borrelia burgdorferi strain B31 is located on the previously undetected circular plasmid cp9-2. J. Bacteriol. 182: 6254-6258. [Abstract]

Stevenson, B., S.F. Porcella, K.L. Oie, C.A. Fitzpatrick, S.J. Raffel, L. Lubke, M.E. Schrumpf, and T.G. Schwan (2000) The relapsing fever spirochete Borrelia hermsii contains multiple, antigen-encoding plasmids that are homologous to the cp32 plasmids of Lyme disease spirochetes. Infect.Immun. 68: 3900-3908. [Abstract]

Bono, J.L., A.F. Elias, J.J. Kupko, B. Stevenson, K. Tilly, and P. Rosa (2000) Efficient targeted mutagenesis in Borrelia burgdorferi. J. Bacteriol. 182: 2445-2452. [Abstract]

Miller, J.C., N. El-Hage, K. Babb, and B. Stevenson (2000) Borrelia burgdorferi B31 Erp proteins that are dominant immunoblot antigens of animals infected with isolate B31 are recognized by only a subset of human Lyme disease patient sera. J. Clin. Microbiol. 38: 1569-1574. [Abstract]

Casjens, S., N. Palmer, R. van Vugt, W.M. Huang, B. Stevenson, P. Rosa, R. Lathigra, G. Sutton, J. Peterson, R.J. Dodson, D. Haft, E. Hickey, M. Gwinn, O. White, and C. Fraser (2000) A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol.Microbiol. 35: 490-516. [Abstract]

El-Hage, N., L.D. Lieto, and B. Stevenson (1999) Stability of erp loci during Borrelia burgdorferi infection: recombination is not required for chronic infection of immunocompetent mice. Infect. Immun. 67: 3146-3150. [Abstract]

Stevenson, B., J.L. Bono, A. Elias, K. Tilly, and P. Rosa (1998) Transformation of the Lyme disease spirochete Borrelia burgdorferi with heterologous DNA. J. Bacteriol. 180: 4850-4855. [Abstract]

Stevenson, B., J.L. Bono, T.G. Schwan, and P. Rosa (1998) Borrelia burgdorferi Erp proteins are immunogenic in mammals infected by tick bite, and their synthesis is inducible in cultured bacteria. Infect. Immun. 66: 2648-2654. [Abstract]

Feng, S., E. Hodzic, B. Stevenson, and S.W. Barthold (1998) Humoral immunity to Borrelia burgdorferi N40 decorin binding proteins during infection of laboratory mice. Infect. Immun. 66: 2827-2835. [Abstract]

Stevenson, B., S. Casjens, and P. Rosa (1998) Evidence of past recombination events among the genes encoding the Erp antigens of Borrelia burgdorferi. Microbiology 144: 1869-1879. [Abstract]

Bono, J.L., K. Tilly, B. Stevenson, D. Hogan, and P. Rosa (1998) Oligopeptide permease in Borrelia burgdorferi: putative peptide binding components encoded by both chromosomal and plasmid loci. Microbiology 144: 1033-1044. [Abstract]

Stevenson, B., S. Casjens, R. van Vugt, S.F. Porcella, K. Tilly, J.L. Bono, and P. Rosa (1997) Characterization of cp18, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids. J. Bacteriol. 179: 4285-4291. [Abstract]

Tilly, K., S. Casjens, B. Stevenson, J.L. Bono, D.S. Samuels, D. Hogan, and P. Rosa (1997) The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene. Mol. Microbiol. 25: 361-374. [Abstract]

Casjens, S., R. van Vugt, K. Tilly, P.A. Rosa, and B. Stevenson (1997) Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes. J. Bacteriol. 179: 217-227. [Abstract]

Rosa, P., D.S. Samuels, D. Hogan, B. Stevenson, S. Casjens, and K. Tilly (1996) Directed insertion of a selectable marker into a circular plasmid of Borrelia burgdorferi. J. Bacteriol. 178: 5946-5953. [Abstract]

Stevenson, B., K. Tilly, and P.A. Rosa (1996) A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31. J. Bacteriol. 178: 3508-3516. [Abstract]

Stevenson, B., T.G. Schwan, and P.A. Rosa (1995) Temperature-related differential expression of antigens in the Lyme disease spirochete, Borrelia burgdorferi. Infect. Immun. 63: 4535-4539. [Abstract]

Stevenson, B., and S.W. Barthold (1994) Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi. FEMS Microbiol. Lett. 124: 367-372. [Abstract]

Stevenson, B., L.K. Bockenstedt, and S.W. Barthold (1994) Expression and gene sequence of outer surface protein C of Borrelia burgdorferi reisolated from chronically infected mice. Infect. Immun. 62: 3568-3571. [Abstract]

 

Edited Books:

Current Protocols in Microbiology (2005-present) edited by R. Coico, T.F. Kowalik, J.M. Quarles, B. Stevenson, and R. Taylor. Wiley and Sons Publishers, Hoboken, N.J. [Article]

 

Invited Reviews:

Kraiczy, P, and B. Stevenson (2013) Complement regulator-acquiring surface proteins of Borrelia burgdorferi: Structure, function and regulation of gene expression, Ticks Tick Borne Dis. 4:26-34 (Abstract)

Verma, A., B. Stevenson, and B. Adler (2013) Leptospirosis in horses, Vet. Microbiol. 167:61-66. (Abstract)

Radolf, J.D, M. Caimano, B. Stevenson, and L. Hu (2012) Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes. Nature Rev. Microbiol. 10: 87-99. (Abstract)

Verma, A., and B. Stevenson (2012) Leptospiral uveitis ­ there is more to it than meets the eye!  Zoonoses Pub. Health 59 (S2): 132-141. (Abstract)

Brissette, C.A., A.E. Cooley, L.H. Burns, S.P. Riley, A. Verma, M.E. Woodman, T. Bykowski, and B. Stevenson (2008) Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection. Int. J. Med. Microbiol. 298 (S1): 257-267.

Bykowski, T., M.E. Woodman, A.E. Cooley, C.A. Brissette, R. Wallich, V. Brade, P. Kraiczy, and B. Stevenson (2008) Borrelia burgdorferi complement regulator-acquiring surface proteins (BbCRASPs): expression profiles during the Lyme disease spirochete¹s mammal-tick infection cycle.  Int. J. Med. Microbiol. 298 (S1): 249-256.

von Lackum, K., K. Babb, S.P. Riley, R.L. Wattier, T. Bykowski, and B. Stevenson (2006) Functionality of Borrelia burgdorferi LuxS: the Lyme disease spirochete produces and responds to the pheromone autoinducer-2, and lacks a complete activated-methyl cycle. Int. J. Med. Microbiol. 296 (S1): 92-102. [Abstract]

Stevenson, B., K. von Lackum, S.P. Riley, A.E. Cooley, M.E. Woodman, and T. Bykowski (2006) Evolving models of Lyme disease spirochete gene regulation. Wien. Klin. Wochenschr. 118: 643-652.

Stevenson, B., K. von Lackum, R.L. Wattier, J.D. McAlister, J.C. Miller, and K. Babb (2003) Quorum sensing by the Lyme disease spirochete. Microbes Infect. 5: 991-997. [Abstract]

Stevenson, B. (2001) Borrelia burgdorferi: a (somewhat) clonal bacterial species. Trends Microbiol. 9:471.

Stevenson, B., W.R. Zückert, and D.R. Akins (2000) Repetition, conservation, and variation: the multiple cp32 plasmids of Borrelia species. J. Mol. Microbiol. Biotechnol. 2:411-422. [Abstract]

Rosa, P., J. Bono, A. Elias, J. Errett, J. Kupko, B. Stevenson, G. Taylor, and K. Tilly (1998) Genetic studies in Borrelia burgdorferi. Wien. Klin. Wochenschr. 110:859-862. [Abstract]

 

Book Chapters:

Riley, S.R., M.E. Woodman, B.L. Jutras, and B. Stevenson (2012) Culture of Escherichia coli and related bacteria.   In Current Protocols in Essential Laboratory Techniques, Second Edition,  S. Gallagher and E. Wiley (eds.)  J. Wiley and Sons, Hoboken, N.J., pp. 4.2.1-27.

Bykowski, T., A. Verma, C.A. Brissette, and B. Stevenson (2012) Aseptic technique. In Current Protocols in Essential Laboratory Techniques, Second Edition, S. Gallagher and E. Wiley (eds.)  J. Wiley and Sons, Hoboken, N.J., pp. 4.1.1-12.

Bykowski, T., and B. Stevenson (2008) Aseptic technique. In Current Protocols in Microbiology.  R. Coico, T.F. Kowalik, J.M. Quarles, B. Stevenson, and R. Taylor (eds.)  J. Wiley and Sons, Hoboken, N.J.,  Appendix 4A.

Riley, S.R., M.E. Woodman, and B. Stevenson (2007) Culture of Escherichia coli and related bacteria.   In Current Protocols in Essential Laboratory Techniques, S. Gallagher and E. Wiley (eds.)  J. Wiley and Sons, Hoboken, N.J., pp. 4.2.1-25.

Bykowski, T., and B. Stevenson (2007) Aseptic technique. In Current Protocols in Essential Laboratory Techniques, S. Gallagher and E. Wiley (eds.)  J. Wiley and Sons, Hoboken, N.J., pp. 4.1.1-11.

Stevenson, B., T. Bykowski, A.E. Cooley, K. Babb, J.C. Miller, M.E. Woodman, K. von Lackum, and S.P. Riley (2006) The Lyme disease spirochete Erp protein family: structure, function and regulation of expression. In Molecular Biology of Spirochetes. F.C. Cabello, H.P. Godfrey and D. Hulinska (eds.). IOS Press BV, Amsterdam, The Netherlands, pp. 354-372 [Link]

Wallich, R., P.F. Zipfel, C. Skerka, M. Kirschfink, M.M. Simon, B. Stevenson, S.M. Lea, and P. Kraiczy (2006) Lyme disease spirochetes evade innate immunity by acquisition of complement regulators. In Molecular Biology of Spirochetes. F.C. Cabello, H.P. Godfrey and D. Hulinska (eds.). IOS Press BV, Amsterdam, The Netherlands, pp. 373-382. [Link]

Stevenson, B. (2005) Common bacterial culture techniques and media. In Current Protocols in Microbiology. R. Coico, T.F. Kowalik, J.M. Quarles, B. Stevenson, and R. Taylor (eds.) J. Wiley and Sons, Hoboken, N.J., pp. A.4A.1

Stevenson, B., W.R. Zückert, and D.R. Akins (2001) Repetition, conservation, and variation: the multiple cp32 plasmids of Borrelia species. In The Spirochetes: Molecular and Cellular Biology. M.H. Saier and J. Garcia-Lara (eds.) Horizon Scientific Press, Norfolk, UK. pp. 87-100

Rosa, P., B. Stevenson, and K. Tilly (1999) Genetic methods in Borrelia and other spirochaetes. In Methods in Microbiology volume 29: Genetic Methods for Diverse Prokaryotes. M.C.M. Smith and R.E. Sockett (eds.) Academic Press, London. pp. 209-227